Product Description:
Biotin-14-dCTP is a dCTP analog which contains biotin attached at the 6 -position of the purine base
by a 14-atom spacer arm. The biotin - labeled nucleotide is incorporated into DNA by nick translation
in the presence of the deoxynucleotide triphosphates dTTP,dGTP and dATP. Other labeling
procedures (i.e., homopolymer, tailing with a terminal deoxynucleotidyl transferase, replacement
synthesis with T4 DNA polymerase or random primed synthesis) may be satisfactory. The
biotin-labeled DNA can be detected colorimetrically using Steptavidin-Alkaline Phosphatase
Conjugate and NBT/BCIP or by chemiluminescence, using streptavidin alkaline phosphatase and an
appropriate chemiluminescent substrate.
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Quality Control:
Purity of biotin-14-dCTP is evaluated by reverse phase HPLC. A single peak with >90% of the area
must be observed.
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